hVEGFA/MC-38
CBPG0239
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产品描述
产品数据库
| I. Background | |
| VEGFA（血管内皮生长因子A）属于PDGF/VEGF 生长因子家族，定位于人类6 号染 色体p21 .1，编码的肝素结合蛋白以二硫键连接的同源二聚体形式存在，是调控血管生成的 核心分子。该基因顺利获得可变剪接产生多个功能各异的异构体，其中VEGF₁₆₅是生物活性最 强的亚型，兼具组织扩散性与靶标结合活性，在生理过程中发挥核心作用。 VEGFA 可结合VEGFR1、VEGFR2 及NRP1 等受体，启动下游多条信号通路，诱导血管内 皮细胞增殖、迁移，抑制内皮细胞凋亡，同时显著提升血管通透性，是胚胎血管发育、成 体组织修复、黄体形成等生理过程的必需调控因子。 | |
| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | VEGFA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right flank region) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hVEGFA/MC38 Mean Body Weight ± SEM.
2. Tumor Volume
Figure 3. hVEGFA/MC38 Mean Tumor Volume ± SEM.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
